A MICROFLUIDIC PERFUSION PLATFORM FOR CULTIVATION AND SCREENING STUDY

A MICROFLUIDIC PERFUSION PLATFORM FOR CULTIVATION AND SCREENING STUDY
SUPPLEMENTAL INFORMATION A MICROFLUIDIC CHIP FOR DETECTING CHOLANGIOCARCINOMA CELLS



A microfluidic perfusion platform for cultivation and screening study of motile microalgal cells

Young-Jae Eu,1,2,a) Hye-Sun Park1, 2,a) and Dong-Pyo Kim2,b) Jong Wook Hong3,4,b)

1Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764, Korea

2National Center of Applied Microfluidic Chemistry, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Gyungbuk, 790-784, Korea

3Materials Research and Education Center, Department of Mechanical Engineering, Auburn University, Auburn AL, 36830, USA

4Department of Bionano Engineering, Hanyang University, Ansan, 427-791, Korea.

a)Y.-J. Eu and H.-S. Park contributed equally to this work.

b)Authors to whom correspondence should be addressed. Electronic mail: [email protected] or [email protected].







A MICROFLUIDIC PERFUSION PLATFORM FOR CULTIVATION AND SCREENING STUDY

Fig. S1. The perfusion barrier of 2 m thickness was produced with SU-8 2 in the first photolithography. The second photolithography was conducted with SU-8 50 to produce the right part of the fluidic channel with no valve function. A positive photoresist, AZ4620 was used in the last step to produce the chamber and channel with valve function. The simple scheme of the used photomask for each photolithography step is shown at the bottom line, and the photomasks (left, middle) for negative photoresist are presented by inverting the black/white. The enlarged photomask pattern for perfusion barrier was shown in the inset.



A MICROFLUIDIC PERFUSION PLATFORM FOR CULTIVATION AND SCREENING STUDY



Fig. S2. A pattern of photomask for fabricating pneumatic valves





A MICROFLUIDIC PERFUSION PLATFORM FOR CULTIVATION AND SCREENING STUDY

Fig. S3. PDMS based microfluidic perfusion chip fabricated by multilayer soft lithography method.





A MICROFLUIDIC PERFUSION PLATFORM FOR CULTIVATION AND SCREENING STUDY



Fig. S4. Flow controller with combination of valves regulates the perfusion of medium into the microchamber array. Flows of two different solutions (middle) or single solution were visualized using water and fluorescent dye solution.



A MICROFLUIDIC PERFUSION PLATFORM FOR CULTIVATION AND SCREENING STUDY



























Fig. S5. Comparative perfusion kinetics of fluorescent dye into perfusion chamber with (a) symmetric channel width (90 m) and (b) asymmetric channel width (30 and 90 m) of inlet and outlet. The black and blue lines are the changes of the relative fluorescence intensities of the fluidic channel and chamber, respectively.







A MICROFLUIDIC PERFUSION PLATFORM FOR CULTIVATION AND SCREENING STUDY



Fig. S6. Growth curve of Chlamydomonas cells in the TAP media in the N-replete and N-starved conditions cultivated in bulk or microfluidic perfusion chamber. The cells were cultivated in shaking flask or in microfluidic perfusion chamber under light of 2000 lux at room temperature. The growth in bulk was measured using absorbance at 600 nm and normalized to the maximum growth. The relative cell growth was shown in linear (a) and log scale (b). Shown is the representative growth curve of three independent experiments.













Tags: cultivation and, screening, platform, perfusion, cultivation, microfluidic, study