VBE MINIMAL AGAR PLATES MEDIUM IS MADE FROM GLUCOSE

100 HIPERMÍNIMOS 100 HYPERMINIMALS FEDERICO SORIANO EL TRABAJO DIARIO
3 MINIMALUS BAUDŽIAMOSIOS ATSAKOMYBĖS AMŽIUS EUROPOS ŠALYSE (2014 M)1
3 ZAŁĄCZNIK NR 3 MINIMALNE WYMAGANIA FUNKCJONALNOTECHNICZNE JAKIE MUSI

45110 Standardna Minimalna Pravila za Alternativne Kaznene Mjere (tokijska
ANEXA IIA FIŞA DE VERIFICARE A ÎNDEPLINIRII STANDARDELOR MINIMALE
ANEXA NR 4 LA REGULAMENT LISTA DOTĂRILOR MINIMALE CORESPUNZĂTOARE

VBE- Minimal Agar Plates

Medium is made from glucose, agar and Vogel-Bonner medium E (50X)

Vogel-Bonner Medium E, 50X (50X VBE)

Reagent	Quantiy
DI water (45 º C)	670 mL
MgSO₄ (7 H₂O)	10 g
Citric Acid monohydrate	100 g
K₂HPO₄ (anhyd.)	500 g
NaNH₄PO₄	175 g

Add salts and citric acid to warm water in a 2 L flask on stirring hotplate in order listed. Wait for each to dissolved completely before adding the next. Bring volume up to 1 L, loosely cap and autoclave.

VBE/glucose agar plates

Reagent	Quatity
Agar	15 g
DI water	930 mL
50X VBE	20 mL
40% glucose	50 mL

Add agar and magnetic stir bar to DI in 2 L flask and bring to boil on hotplate stirrer. Autoclave. Sterile filter 40% glucose and 50X VBE. Add VBE and glucose to agar solution using sterile technique (in a sterile field) cover flask and stir. Pour plates when soln. is mixed.

Soft Agar Overlays

Reagent	Quatity
DI water	340 mL
VBE/glucose agar soln.	200 mL
NaCl	3 g
Trace stock soln.	60 mL

Added components to 2 L flask on hot plate stirrer and mix.

Aliquot and autoclave.

Trace stock solution

Reagent	Quatity
Biotin	6.1 mg
Histidine	5.25 mg
DI water	100 mL

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Tags: glucose 50, and glucose, medium, glucose, plates, minimal