DNA SEQUENCING RESULTS NOISY DATA BACKGROUND NOISE APPEARS

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AGTC SAMPLE SUBMISSION STORAGE AND RETRIEVAL POLICY SEQUENCING TEMPLATES
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BROWNsequencingBJHPversion3
CLASSIFICATION BIOLOGICAL SCIENCE SYSTEMS BIOLOGY DIGITAL RNA SEQUENCING MINIMIZES
DNA SEQUENCING RESULTS MIXED SIGNALS MULTIPLE PEAKS ON TOP

Noisy Sequence

DNA sequencing results: Noisy data

Background noise appears as many smaller, undefined peaks under your sequence peaks of interest. This noise is always present, but with well-prepared samples of good signal strength, it will be undetectable.

If the noises are too strong, the software has difficulty in resolving the base peaks from background noise. This results in poor sequence reliability

DNA SEQUENCING RESULTS NOISY DATA BACKGROUND NOISE APPEARS

Example of noisy data: presence of Background noise misleading the automated base caller at various points in the chromatogram

Causes of Noisy Sequence

Dirty DNA

Low DNA concentration. As the signal intensity is low, the background noise will be artificially amplified by the software in order to called bases

Inadequate primers:

Inefficient primer annealing

Multiple priming sites

Noisy data caused by random priming of a N-1 primer synthesis

Possible solutions

Increase template concentration

Re-do DNA prep

Request alternative sequencing programmes

Choose another primer

DNA SEQUENCING RESULTS NOISY DATA BACKGROUND NOISE APPEARS
PORCINE SEQUENCING WHITE PAPER PORCINE GENOMIC SEQUENCING INITIATIVE
REDUCED REPRESENTATION SHOTGUN SEQUENCING (RRS) GENOMIC DNA WAS ISOLATED

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