TABLE S1 YEAST STRAINS STRAIN BACKGROUND RELEVANT GENOTYPE AGGREGATION

TABLE OF CONTENTS TO MAKE IT
ROUND TABLE ON SUSTAINABLE DEVELOPMENT ANNOTATED AGENDA MEETING
REQUIRED COMMANDS FOR WRITABLE CDDVD DEVICES — 29 WINDOWS

0 – TABLE OF CONTENTS PAGE PRACTISING LAW
19 Table Ronde La Libéralisation du Commerce
23 BALANCE OF PAYMENTS STATISTICS METHODOLOGY TABLE

Strain

Table S1 Yeast strains

Strain	Background	Relevant genotype	Aggregation phenotype*	Reference
YH1A	S288C	MATa ho::kanMX4	1	Hu, et al. 2007 [15]
YH1C	S288C	MATα ho::kanMX4	1	Hu, et al. 2007 [15]
YL1C	YL1C	MATα ho::kanMX4	3	Hu, et al. 2007 [15]
YL1A	YL1C	MATa ho::kanMX4	3	Hu, et al. 2007 [15]
S2	F1 segregant of YH1A×YL1C	MATα ho::kanMX4	4	Hu, et al. 2007 [15]
S5	F1 segregant of YH1A×YL1C	MATα ho::kanMX4	4	Hu, et al. 2007 [15]
S27	F1 segregant of YH1A×YL1C	MATa ho::kanMX4	1	Hu, et al. 2007 [15]
S57	F1 segregant of YH1A×YL1C	MATa ho::kanMX4	4	Hu, et al. 2007 [15]
S67	F1 segregant of YH1A×YL1C	MATa ho::kanMX4	3	Hu, et al. 2007 [15]
S95	F1 segregant of YH1A×YL1C	MATα ho::kanMX4	1	Hu, et al. 2007 [15]
S116	F1 segregant of YH1A×YL1C	MATa ho::kanMX4	4	Hu, et al. 2007 [15]
S123	F1 segregant of YH1A×YL1C	MATα ho::kanMX4	3	Hu, et al. 2007 [15]
S150	F1 segregant of YH1A×YL1C	MATa ho::kanMX4	2	Hu, et al. 2007 [15]
S152	F1 segregant of YH1A×YL1C	MATa ho::kanMX4	2	Hu, et al. 2007 [15]
YL1C-amn1⊿	YL1C	MATα ho::kanMX4 amn1::HYGB	1	This study
YL1A-amn1⊿	YL1C	MATa ho::kanMX4 amn1::HYGB	1	This study
YL1C-flo8⊿	YL1C	MATα ho::kanMX4 flo8::HYGB	2	This study
YL1C-rga1⊿	YL1C	MATα ho::kanMX4 rga1::HYGB	2	This study
YL1C-flo1⊿	YL1C	MATα ho::kanMX4 flo1::HYGB	2	This study
YL1C- flo8⊿&rga1⊿	YL1C	MATα ho::kanMX4 flo8::HYGB rga1::NAT	2	This study
YL1C- flo8⊿&RGA1^T367P	YL1C	MATα ho::kanMX4 flo8::HYGB rga1⊿::RGA^T367P -YL1C^$	Not assayed	This study
YL1C- flo8⊿&RGA1^F415I	YL1C	MATα ho::kanMX4 flo8::HYGB rga1⊿::RGA^F415I-YL1C^$	Not assayed	This study
YL1C- flo8⊿&RGA1^S658Y	YL1C	MATα ho::kanMX4 flo8::HYGB rga1⊿::RGA^S658Y-YL1C^$	Not assayed	This study
YL1C- flo8⊿&RGA1^A866V	YL1C	MATα ho::kanMX4 flo8::HYGB rga1⊿::RGA^A866V-YL1C^$	Not assayed	This study
YL1C- amn1⊿&rga1⊿	F1 segregant of YL1A-amn1⊿×YL1C- flo8⊿&rga1⊿	MATα ho::kanMX4 amn1::HYGB rga1::NAT	2	This study
YL1C- amn1⊿&flo8⊿	F1 segregant of YL1A-amn1⊿×YL1C- flo8⊿&rga1⊿	MATα ho::kanMX4 amn1::HYGB flo8:: HYGB	1	This study
YL1C- amn1⊿&flo8⊿&rga1⊿	F1 segregant of YL1A-amn1⊿×YL1C- flo8⊿&rga1⊿	MATα ho::kanMX4 flo8:: HYGB amn1::HYGB rga1::NAT	1	This study
YL1C-AMN1^A	YL1C	MATα ho::kanMX4 amn1⊿::AMN1-YH1A	1	This study
YL1C-FLO8^A	YL1C	MATα ho::kanMX4 flo8⊿::FLO8-YH1A	2	This study
YL1C-RGA1^A	YL1C	MATα ho::kanMX4 rga1⊿::RGA1-YH1A	2	This study
YH1A-AMN1^C	YH1A	MATα ho::kanMX4 amn1⊿::AMN1-YL1C	2	This study
YH1A-flo1⊿	YH1A	MATα ho::kanMX4 flo1::HYGB	1	This study
YH1A-flo8⊿	YH1A	MATα ho::kanMX4 flo8::HYGB	1	This study
S2-flo1⊿	S2	MATα ho::kanMX4 flo1::HYGB	2	This study
S5-flo1⊿	S5	MATα ho::kanMX4 flo1::HYGB	2	This study
YL1C-AMN1^R226Q	YL1C	MATα ho::kanMX4 amn1⊿:: AMN1^R226Q-YH1A^#	1	This study
YL1C-AMN1^H233R	YL1C	MATα ho::kanMX4 amn1⊿:: AMN1^H233R-YH1A^#	1	This study
YL1C-AMN1^V368D	YL1C	MATα ho::kanMX4 amn1⊿:: AMN1^V368D-YH1A^#	3	This study
AH109	host strain of yeast two hybrid	MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4 gal80 LYS2::GAL1_UAS-GAL1_TATA-HIS3 GAL2_UAS-GAL2_TATA-ADE2 URA3::MEL1_UAS-MEL1_TATA-lacZ	Not assayed	James et al, 1996 [22]
Y187	host strain of yeast two hybrid	MATα ura3-52 his-200 ade2-101 trp1-901 leu2-3,112 gal4 met gal80 URA3::GAL_UAS-GAL1_TATA-lacZ	Not assayed	Harper et al, 1993 [23]

*: 1-4 represent the four categories of aggregation phenotype

#: AMN1^XXXXX-YH1A represents a YH1A-derived AMN1 allele containing xxxxx mutation. For example, AMN1^R226Q-YH1A is a YH1A-derived AMN1 allele containing R226Q mutation.

^$ RGA1^XXXXX-YL1C represents a YL1C-derived RGA1 allele containing xxxxx mutation. For example, RGA1^T357P-YL1C is a YL1C-derived RGA1 allele containing T357P mutation.

Table S2. The 25 SNP markers add for high-resolution QTL mapping.

Chr.	Marker Name	Location	Forward primer	Backward primer	Enzyme
1	FLO1	203630	CTCATCGCTATATGTTTTTGG	CGAGTAAACAACCTTCATTGG	EcoRV
2	YBR137	513196	ATTGGAAAGATTGACTTCTCG	GATTGTATTAGGACAGCACCTC	ApoI
2	YBR149	540259	AGCCGCTCTACCCTTACCTC	CTTGGTTCACCGTTGGCTTA	BglⅡ
2	YBR153	547627	GATGGGAATACCAAATACGACG	ATTTATTACATTGGCACCTCCT	AvaI
2	YBR158	557219	CTCAACATCAGCGAAGTCAT	TCCTTAAAATTATGGACGCT	BsaI
2	YBR168	572991	GGCGTCAATAAGGAGTGTTC	ACAGCAGTAAACCTCCAAAG	MspI
2	YBR180	589832	TGATAAGGCTAGAAGGATGGT	TTGAGTATCAGGAGAAGGGTC	BglⅡ
2	YBR189	604415	CGTAACTGATTTAATTCTTGGTC	GGAAAATAAAATACTGTCCCAT	Mph1103I
2	YBR201	623814	TTAGGCGACATTCCTTTGGTC	ACGTTCATTTGGTTTCCGTTC	HinCⅡ
2	YBR211	646858	TATTTAAGACGCCAAATGCTGT	ATCGAAATTGTAACGAGTAGTAAGG	MboI
5	8s5	333160	GGAGTATGGTAGGAGGTAGA	TACATCCATCACTACCGAAAC	MseI
5	FLO8	377244	CAATGGCAACGAATAGTGA	TGTATGGTGGAATGGAAAA	AccI/SalI
5	11s5	467719	TATCCACCGTTATTCTCCC	GACCTTCAAATAAATACTCGC	TaiI
5	12s5	517939	GACGCCAGCACTTTCCAG	GGTGGTGACTATTCTTCGGCA	TaaI
15	YOR096	505528	CGTCATTCATTTCAAACCCAT	CATTCTGGTATCGTCCCGTTC	HaeIII
15	YOR106	518802	AGGTGCCTTCTAACAGGTAACAC	GCTGAACGAATATTATCATTTGA	BseGI
15	YOR119	550196	AGCGGATTTCTGGGACTTTAG	TACCTGCAAAAGCGTGGTAAA	AvaII
15	YOR127	562241	TAACGATAGCGATGACAACTCTG	AACGTTACTATCCTGTTTGCCTG	BamHI
15	YOR138	586852	TGCTGTCTTAGGACCCTCTGAAT	TAGTGAACCTTCATCGCCACAG	NdeI
15	YOR143	602416	GATTTGGTCTATCAGTTGTCACA	TTTTCAATACACTCCTCGCTCAT	BfmI
15	YOR153	619601	TTCCTACTTATGGTAATGTGCTA	AAGGTATGATAATAAAAGCGAAC	BsrGI
15	YOR161	639042	CTTGATGAAGGATAAGCATACGC	TGCAGTAATTAGCCGTAGTGGAC	BsrBI
15	YOR168	651538	AGGAGACTCCTTCGGACTATCAA	CCTCGCCATTAGCCTTTACAT	BglII
15	YOR175	661971	TAAACCGACTCAAGTGGAACA	AGCAGATAGCATATCGCAGTGTA	ApoI
15	YOR185	682563	ACTTTATCCAGGGAATTATCGA	ACTTTATCCAGGGAATTATCGA	BstBI

ANSØGNINGSSKEMA GODKENDELSE TIL AT ETABLERE OG DRIVE PRIVAT
APPLICATION TO SALARY SACRIFICE A PORTABLE ELECTRONIC DEVICE
◄BACK TO TABLE OF CONTENTS INTERVENTION STRATEGY BUILDING

Tags: aggregation phenotype*, of aggregation, background, yeast, table, genotype, aggregation, strains, strain, relevant