NAME LIBERTY SCIENCE CENTER FIND THE LIMITING NUTRIENT

ADAM SMITH PRESUMPTIONOFLIBERTY QUOTATIONS COLLECTED BY DANIEL KLEIN (NOTATION
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CONSTITUTION OF THE YOUNG AMERICANS FOR LIBERTY THE OHIO
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Define “Limiting Nutrient” – show diagram or physical model – for phytoplankton to grow they need the correct amount of each;

Name: ________________________

LIBERTY SCIENCE CENTER

Find the Limiting Nutrient

MEASUREMENTS: With your group, measure each water sample for each nutrient. Be sure to record the names/where your three water samples are from!

TABLE 1 – RECORD YOUR MEASUREMENTS

Sample name

1)

2)

3)

Nitrate (NO3)




Phosphate (PO4)




Iron (Fe)





Scientists found that the ratio of these nutrients in the ocean looks something like this:

N : P : Fe =

16 : 1 : 0.25

This ratio is called the Redfield ratio, named after the scientist who discovered it.

EXAMPLES: Find the limiting nutrient!

The limiting nutrient is the one plants will use up first. Compare this to the ratio above:

17 : 1 : 0.1

Can you tell which nutrient is limiting?







Using the following chart, you’ll compare measured nutrients to their Redfield ratio numbers. The answer is how many “units” of plants you could build. (We multiply by 100 to make the numbers easier to compare.) The nutrient that can build the least amount of plants is limiting.

EXAMPLE 1 – complete the chart

[A]

Measured

[B]

Measured X100


Redfield #

[C]

[B] /Redfield# = “plant units”

Nitrate (NO3)

1

100

16

100/16 = 6.25

Phosphate (PO4)

0.4

40

1


Iron (Fe)

0.01


0.25



Which nutrient is most limiting? ___________________

Which nutrient has the most excess? _________________



EXAMPLE 2 – complete the chart

[A]

Measured

[B]

Measured X100


Redfield #

[C]

[B] /Redfield# = “plant units”

Nitrate (NO3)

20


16


Phosphate (PO4)

0.2


1


Iron (Fe)

0.09


0.25




Which nutrient is most limiting? ___________________

Which nutrient has the most excess? _________________

CALCULATIONS: Now find the limiting nutrient for your own water samples. Look up your nutrient values in Table 1 and use the following charts to find which nutrient is limiting.

SAMPLE 1: __________________

[A]

Measured

[B]

Measured X100


Redfield #

[C]

[B] /Redfield# = “plant units”

Nitrate (NO3)



16


Phosphate (PO4)



1


Iron (Fe)



0.25




Which nutrient is most limiting? ___________________

Which nutrient has the most excess? _________________



SAMPLE 2: __________________

[A]

Measured

[B]

Measured X100


Redfield #

[C]

[B] /Redfield# = “plant units”

Nitrate (NO3)



16


Phosphate (PO4)



1


Iron (Fe)



0.25




Which nutrient is most limiting? ___________________

Which nutrient has the most excess? _________________

SAMPLE 3: __________________

[A]

Measured

[B]

Measured X100


Redfield #

[C]

[B] /Redfield# = “plant units”

Nitrate (NO3)



16


Phosphate (PO4)



1


Iron (Fe)



0.25




Which nutrient is most limiting? ___________________

Which nutrient has the most excess? _________________



QUESTIONS

Was the same nutrient limiting in all your samples?

Think about where your water samples came from. Can you think of any reasons why the limiting nutrient is what it is?











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