CHAPTER 3 QUESTION 3 USE THE CODON DICTIONARY (FIGURE

CHAPTER 11 OECD AVERAGE AND OECD TOTAL BOX
 CONTENTS PREFACE IX INTRODUCTION 1 REFERENCES 5 CHAPTER
 NRC INSPECTION MANUAL NMSSDWM MANUAL CHAPTER 2401 NEAR‑SURFACE

32 STAKEHOLDER ANALYSIS IN THIS CHAPTER A STAKEHOLDER ANALYSIS
CHAPTER 13 MULTILEVEL ANALYSES BOX 132 STANDARDISATION OF
CHAPTER 6 COMPUTATION OF STANDARD ERRORS BOX 61

Chapter 3:

Chapter 3:

Question #3:

Use the codon dictionary (Figure 3-18 in text) to complete the following table. Assume that reading is from left to right and that the columns represent transcriptional and translational alignments.


▪While completing table, be sure and label the 5’ and 3’ ends of the DNA and RNA as well as the amino and carboxy ends of the protein


3’

C

G

T

A

C

C

A

C

T

G

C

A

DNA Double Helix

5’

G

C

A

T

G

G

T

G

A

C

G

T


5’

G

C

A

U

G

G

U

G

A

C

G

U

mRNA transcribed

3’

C

G

U

A

C

C

A

C

U

G

C

A

Appropriate tRNA anticodon

Amino




Ala




Trp




Stop





Amino acid incorporated into protein

This question definitely gets you to think about the relationship between DNA, mRNA, and proteins produced…See Figure 3-2 in text


Question #11:

A certain Drosophila protein-coding gene has one intron. If a large sample of null alleles of the gene is examined, will any of the mutant sites be EXPECTED:

First, what is a null mutation?! A null mutation is one that results in complete absence of function of the geneResults in no protein function (see page 72 in text)


a.) in the exons? So, ask yourself—Can a mutation occur in the exon that would result in creation of a non-functional protein? YES! A missense, nonsense, deletion, or frameshift could occur that would disrupt the reading frame resulting in a non-functional protein


b.) in the introns? Generally speaking…No. BUT! There are those CRITICAL consensus splice junction sequences at the exon-intron boundary that are necessary for proper splicing! So, the book says Yes.


c.) in the promoter? Ask yourself—What acts at the promoter?! RNA Polymerase…Okay, there are some critical regions in the promoter (namely –10 and –35) that serve as binding sites for RNA Polymerase. If those were mutated, could that possibly result inproduction of a non-functional protein? YES! Mutation at a promoter may disrupt the binding of RNA Polymerase. So where once a protein SHOULD have been produced, now NO protein is produced…


d.) in the exon-intron boundary? See “b” YES! Disruption of consensus splice sites may lead to production of truncated proteins or proteins that are longer than they should be.


The POINT of this question is best represented in Figure 3-25 of your text—Mutant sites have different consequences!!


Question #23:

List three examples of proteins that act on nucleic acids…

With what nucleic acids are we familiar? DNA and RNA! So, what proteins bind to DNA and RNA?? Don’t forget that an enzyme is a protein!

-histones, DNA/RNA polymerases, topoisomerase, ligase, restriction enzymes, ribosomal proteins (to name a few…)


CONFIGURING USER STATE MANAGEMENT FEATURES 73 CHAPTER 7 IMPLEMENTING
INTERPOLATION 41 CHAPTER 5 INTERPOLATION THIS CHAPTER SUMMARIZES POLYNOMIAL
PREPARING FOR PRODUCTION DEPLOYMENT 219 CHAPTER 4 DESIGNING A


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